|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Monolayer formation of human osteoblastic cells on vertically aligned multiwalled carbon nanotube scaffolds
Anderson O Lobo*†1, Erica F Antunes*†, Mariana BS Palma*, Cristina Pacheco‑Soares‡, Vladimir J Trava‑Airoldi*† and Evaldo J Corat*†
*Laboratrio Associado de Sensores e Materiais, Instituto Nacional de Pesquisas Espaciais, CP 515, So Jos dos CamposSP, CEP 12.245970, Brazil, †Instituto Tecnolgico de AeronuticaCTA, So Jos dos CamposSP, CEP 12228900, Brazil, and ‡Laboratrio de Dinmica de Compartimentos Celulares, Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraba, So Jos dos CamposSP, CEP 12227010, Brazil
Monolayer formation of SaOS-2 (human osteoblast-like cells) was observed on VACNT (vertically aligned multiwalled carbon nanotubes) scaffolds without purification or functionalization. The VACNT were produced by a microwave plasma chemical vapour deposition on titanium surfaces with nickel or iron as catalyst. Cell viability and morphology studies were evaluated by LDH (lactate dehydrogenase) release assay and SEM (scanning electron microscopy), respectively. The non-toxicity and the flat spreading with monolayer formation of the SaOs-2 on VACNT scaffolds surface indicate that they can be used for biomedical applications.
Key words: cell adhesion, cytotoxicity, lactate dehydrogenase assay, SaOs-2, vertically aligned multiwalled carbon nanotubes (VACNT)
Abbreviations: CNT, carbon nanotubes, FBS, fetal bovine serum, HMDS, hexamethyldisilazane, LDH, lactate dehydrogenase, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide, MWCVD, microwave plasma chemical vapour deposition, OD, optical density, SaOS-2, human osteoblast-like cells, SEM, scanning electron microscopy, SWCNT, single-walled carbon nanotube, TEM, transmission electron microscopy, VACNT, vertically aligned multiwalled carbon nanotubes
1To whom correspondence should be addressed (email firstname.lastname@example.org).
Received 8 August 2008/19 May 2009; accepted 30 October 2009
Published as Cell Biology International Immediate Publication 30 October 2009, doi:10.1042/CBI20090131
© The Author(s) Journal compilation © 2010 Portland Press Limited