|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Suppression of PTP1B in gastric cancer cells in vitro induces a change in the genome-wide expression profile and inhibits gastric cancer cell growth
Jinguo Wang, Xuehua Chen, Bingya Liu and Zhenggang Zhu1
Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 20025, Peoples Republic of China
PTP1B (protein tyrosine phosphatase 1B) is a member of the superfamily of PTPs (protein tyrosine phosphatases) and has been implicated in cancer pathogenesis. However, the role of PTP1B in gastric cancer is still unknown. Here, we first detected the PTP1B expression in six gastric cancer cell lines and in the immortalized gastric mucosal epithelial cell line GES-1 by RT-PCR and Western blot. Then, we measured the change of the genome-wide expression profile in MKN28 gastric cancer cells transfected with a plasmid expressing PTP1B-specific small interfering RNA by microarray analysis. Our results showed that PTP1B was overexpressed in gastric cancer cells, and inhibition of PTP1B expression dramatically inhibited gastric cancer cell growth in vitro and in vivo. In addition, microarray analysis revealed that inhibition of PTP1B induced changes in the genome-wide expression profile. These changes may be related to cell growth. Taken together, our data suggested that PTP1B may be a candidate oncogene in gastric cancer.
Key words: cell growth, gastric cancer, microarray analysis, proliferation, PTP1B, small interfering RNA
Abbreviations: GO, gene ontology, PTPs, protein tyrosine phosphatases, PTP1B, protein tyrosine phosphatase 1B, qRT-PCR, quantitative real-time PCR, siRNA, small interfering RNA
1To whom correspondence should be addressed (email email@example.com).
Received 22 November 2009/24 February 2010; accepted 14 April 2010
Published as Cell Biology International Immediate Publication 14 April 2010, doi:10.1042/CBI20090447
© The Author(s) Journal compilation © 2010 Portland Press Limited