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Cell Biology International (2010) 34, 893–899 (Printed in Great Britain)
A FANCD2 domain activates Tip60-dependent apoptosis
James Hejna, Donald Bruun1, Daniel Pauw and Robb E Moses2
Department of Molecular and Medical Genetics, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 972393098, U.S.A.


1Present address: 1120 Haring Hall, One Shields Ave., Davis, CA 95616, U.S.A.

2To whom correspondence should be addressed (email mosesr@ohsu.edu).


This supplementary data is also available as a PDF

SUPPLEMENTARY ONLINE DATA



Figure S1 Fluorescence-based assay

(A) Transfection of GM639 fibroblasts with EGFP-exons 10–13 lacking the NLS. Numbers indicate nucleotides. (B) EYFP-exons 10–13 lacking the NLS co-transfected with ECFP-NLS. (C) GM639 fibroblasts co-transfected with ECFP-NLS and DsRed2-C1 plasmids. Left panel, blue filter; right panel, red filter.



Figure S2 Cell survival after Tip60 depletion

Tip60-depleted HeLa cells were co-transfected with EGFP-NLS (left column) or EGFP-NLS-exons 10–13 (right column) and pDsRed2-C1. Control mock-depleted cells were co-transfected with EGFP-NLS-exons 10–13 and pDsRed2-C1-exons 10–13 (middle column). Top row, DAPI (4′,6-diamidino-2-phenylindole); middle row, EGFP; bottom row, DsRed. Apoptotic cells are identified by arrows.


Table S1 Primers used in the present study

Primer Sequence Position
FAD-763F 5′-CACAAAGCTTGACCCAAACTTCCTATTGAAGG-3′ 763
FAD-778F 5′-CACAAAGCTTTTGAAGGTTCGCCAGTTGGTG-3′ 778
FAD-799F 5′-CACAAAGCTTATGGATAAGTTGTCGTCTATTAG-3′ 799
FAD-819F 5′-CACAAAGCTTATTAGATTGGAGGATTTACTGTG-3′ 819
FAD-841F 5′-CACAAAGCTTATAATAAAGTTCATTCTTCATTCCG-3′ 841
FAD-871F 5′-CACAAAGCTTGCCATGGATACACTTGAGG-3′ 871
FAD-898F 5′-CACAAAGCTTGAGCTTCGGGAGAAGTTGG-3′ 898
FAD-1016R 5′-CACAGGATCCCCGCTGCTTTCTTGATTTCC-3′ 1016
FAD-1039R 5′-CACAGGATCCAGAGGAGAATAATACAGCTCTG-3′ 1039
FAD-1057R 5′-CACAGGATCCCTGACTTTATTACATCAAAGAGG-3′ 1057
FAD-1076R 5′-CACAGGATCCGTTTTCTCATATCTAATAGCTGAC-3′ 1076
FAD-1101R 5′-CACAGGATCCTTAATCCAGGCTTCTGAAATGG-3′ 1101
FAD-1245R 5′-CACAGGATCCTGCTGTTCTTGAATGCAGCCTG-3′ 1245



Received 30 September 2009/28 April 2010; accepted 27 May 2010

Published as Cell Biology International Immediate Publication 27 May 2010, doi:10.1042/CBI20090251


© The Author(s) Journal compilation © 2010 Portland Press Limited


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)