Brought to you by Portland Press Ltd.
Published on behalf of the International Federation for Cell Biology
Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
Cell Biology International (2010) 34, 997–1003 (Printed in Great Britain)
Evidence that core histone H3 is targeted to the mitochondria in Brassica oleracea
Mary Katherine B. Zanin*1, Jon M Donohue† and Bryan A Everitt*
*Department of Biology, The Citadel, 171 Moultrie Street, Charleston, SC 294096170, U.S.A., and †Division of Rheumatology and Immunology, Medical University of South Carolina, 171 Ashley Ave., Charleston, SC 29425, U.S.A.


1To whom correspondence should be addressed (email kathy.zanin@citadel.edu).


This supplementary data is also available as a PDF

SUPPLEMENTARY DATA



Figure S1 Western blot of mitochondrial and nuclear extracts using a different primary antibody against the C-terminus of H3, Active Motif 39164

Active Motif 39164, antibody against H3 C-terminus, duplicates data produced with Sigma H0164 in Figure 2 of the main paper. Western blots of nuclear (N) and mitochondrial (M) extracts from B. oleracea: (A) probed with Active Motif 39164 to the C-terminus of H3 (marked with arrowhead). (B) The same strip seen in (A) re-probed with an antibody, Abcam ab4566, to the nucleolar marker fibrillarin (marked *) shows very low level of nuclear contamination of the mitochondrial fraction.



Table S1 List of all proteins identified in LC-MS/MS data from The Rockefeller University Protein/DNA Technology Center, grouped by protein type (organelle localization)

Protein type Protein
Histone protein (nucleus) Histone H3 (Narcissus pseudonarcissus)
Histone H3 protein, putative (Arabidopsis thaliana)
Histone H2A.2.1
Histone H2A (Hyacinthus orientalis)
H2A protein (Oryza sativa)
ATP synthase (mitochondria) Hypothetical protein ymf19 (ORF158)
orfB (Raphanus sativus)
Hypothetical protein ymf19 (ORFB)
ATPase (mitochondria, chloroplast) Putative proteolipid subunit of vacuolar H+ ATPase
Nucleoside diphosphate kinase (mitochondria, chloroplast, cytoplasm) NDPK III (Brassica rapa)
Nucleoside diphosphate kinase 4 (nucleoside diphosphate kinase IV) (NDK IV) (NDP kinase IV) (NDPK IV) (nucleoside diphosphate kinase III)
Nucleoside diphosphate kinase, NDPK = Nm23 protein homologue {N-terminal} {EC 2.7.4.6} (Avena sativa = oats, Garry, Peptide Partial, 24 aa)
Os05g0595400 [Oryza sativa (japonica cultivar-group)]
Calmodulin (ER, cytoplasm) Calmodulin (Solanum lycopersicum)
Calmodulin [Oryza sativa (indica cultivar-group)]
CAM2 (Calmodulin-2); calcium ion binding (Arabidopsis thaliana)
Calmodulin (Arabidopsis thaliana)
Calmodulin (Quercus petraea)
Calmodulin (Aegiceras corniculatum)
Calmodulin (Solanum lycopersicum)
Peroxiredoxin (chloroplast) Peroxiredoxin protein (Arabidopsis thaliana)
Putative defensin-like protein (secreted/ER) 4D11_26 (Brassica rapa subsp. pekinensis)
Dehydration stress-induced protein Dehydration stress-induced protein (Brassica napus)
AT4g39730/T19P19_120 (Dehydration stress-induced protein) putative
Ribosomal proteins (rER, cytoplasm) 40S ribosomal protein S14
Cytoplasmic ribosomal protein S14 (Brassica napus)
40S ribosomal protein (Arabidopsis thaliana)
Structural constituent of ribosome (Arabidopsis thaliana)
S18.A ribosomal protein (Arabidopsis thaliana)
Glycine-rich RNA-binding protein (chloroplast, nucleus, peroxisome) Glycine-rich RNA-binding protein 7 (Arabidopsis thaliana)
FKBP subfamily, peptidylprolyl isomerase (ER) Probable peptidylprolyl isomerase (EC 5.2.1.8) FKBP15-1 – Arabidopsis thaliana
Immunophilin
Other proteins predicted by gene sequence F1E22.17 (Arabidopsis thaliana)
Hypothetical protein B – rape mitochondrion
Putative S-domain receptor-like protein kinase [Oryza sativa (japonica cultivar-group)]




Received 9 October 2009/1 June 2010; accepted 2 July 2010

Published as Cell Biology International Immediate Publication 27 May 2010, doi:10.1042/CBI20090281


© The Author(s) Journal compilation © 2010 Portland Press Limited


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)