|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Sodium butyrate induces differentiation of gastric cancer cells to intestinal cells via the PTEN/phosphoinositide 3-kinase pathway
Zhigang Bai*, Zhongtao Zhang*1, Yingjiang Ye† and Shan Wang†
*Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, Peoples Republic of China, and †Department of Gastroenterological Surgery, Peking University Peoples Hospital, Beijing 100044, Peoples Republic of China
NaB (sodium butyrate) inhibits cell proliferation and induces differentiation in a variety of tumour cells. In this study, we aimed to determine whether NaB induced differentiation and regulated the expression of the mucosal factor MUC2 through the PTEN/PI3K (phosphoinositide 3-kinase) pathway. BGC823 cells treated with NaB for 24–72 h showed marked inhibition of cell proliferation and alteration in cellular morphology. NaB treatment markedly increased the expression of PTEN and MUC2, but it decreased the expression of PI3K. These effects were enhanced by intervention with PI3K inhibitors and were reduced by intervention with PTEN siRNA. Hence, we conclude that NaB increased PTEN expression, promoted the expression of MUC2 and induced the differentiation of gastric cancer cells through the PTEN/PI3K signalling pathway.
Key words: differentiation, gastric cancer, MUC2, PTEN, PI3K, sodium butyrate
Abbreviations: CDS, coding sequence, EGFP, enhanced green fluorescent protein, MUC2, mucin 2, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, NaB, sodium butyrate, PI3K, phosphoinositide 3-kinase, PTEN, phosphatase and tensin homologue deleted on chromosome 10, siRNA, small interfering RNA
1To whom correspondence should be addressed (email email@example.com).
Received 19 December 2009/17 July 2010; accepted 19 August 2010
Published as Cell Biology International Immediate Publication 19 August 2010, doi:10.1042/CBI20090481
© The Author(s) Journal compilation © 2010 Portland Press Limited