|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2010) 34, S8 (Printed in Great Britain)
Co-localization of CD147 and matrix metalloproteinases and its implication for spermatogenesis during mouse testis development
Hao Chen12, Kin Lam Fok2, Yaoting Gui1, Zhiming Cai1 and Hsiao Chang Chan2
1Shenzhen Key Lab of Male Reproduction and Genetics, Peking University Shenzhen Hospital, and 2Epithelial Cell Biology Research Center, Key Laboratory for Regenerative Medicine of Ministry of Education of China, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong
Matrix metalloroteinases (MMPs) are the critical proteinase in the restructuring events during the testis development. CD147, as extracellular matrix metalloproteinase inducer (EMMPRIN), is known to stimulate the production of MMPs in tumor metastasis and its knockout mice an infertile, suggesting its possible involvement in spermatogenesis. We here showed that CD147, both detected by RT-PCR and western blot, was present at all stages of testicular development from day 7 to day 56. Interestedly, CD147 was increased dramatically after 21 days compared to 7 days and 14 days. Of eight MMPs studied, MMP-2, MMP-7, MMP-9 and MMP-23 were detected changes in expression, especially MMP-2 with dynamic change during testicular development. MMP-2, observed by immunofluorescence, was present around the spermatogonia cell and elongated spermtids. Furthermore, CD147 and MMP-2 were co-localized on the surface of spermatogonia. These data suggest that CD147 might regulate germ cell migration via induction of MMP production during spermatogenesis.
Published online 1 August 2010, doi:10.1042/CBI034S008b
© The Author(s) Journal compilation © 2010 Portland Press Limited