|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2010) 34, S18 (Printed in Great Britain)
The effect of lead exposure on DCX positive cells in layer II of adult guinea pigs neocortex
Kun Xiong1, Kai Huang1, Lei Shang1, Hui Wang1, Xiao‑xin Yan2 and Ju‑fang Huang1*
1Department of Anatomy and Neurobiology, Xiang-ya School of Medicine, Central South University, Changsha, Hunan, 410013, and 2Department of Anatomy, School of Medicine, Southern Illinois University, Carbondale, IL 62901, U.S.A.
Lead, as a kind of neurotoxic heavy metal, has been widely explored as for its influence on neural development in the children, however, it is still unveiled that effect of lead exposure on neurogenesis in adult rodent. Our previous data showed that double-cortin (DCX) positive cells (immature neuron) in the layer II of adult guinea pig neocortex, maintained in the whole adulthood, even in the aged guinea pig. In the present study, we tried to investigate the effect of lead exposure on DCX positive cells in layer II of adult guinea pig. The normal adult guinea pigs were given the drinking water which contained lead acetate (0.2 g/L) daily, the exposured animals were sacrificed in 2, 4 and 6 months, the same aged guinea pigs served as control. The neocortex slices were stained by DCX immunohistochemisty and DCX positive cells were counted. We found that density of DCX positive cells in frontal cortex, temporal cortex, parietal cortex and occipital cortex was decreased (P<0.05) as lead exposure period extended. These results show that lead exposure results in loss of immature neurons in adult neocortex, at least, suggest lead exposure could affect neurogenesis in the adulthood negatively.
Published online 1 August 2010, doi:10.1042/CBI034S018d
© The Author(s) Journal compilation © 2010 Portland Press Limited