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Cell Biology International (2010) 34, S26 (Printed in Great Britain)
Effects of marginal vitamin A deficiency beginning from pregnancy on hippocampus synaptic plasticity related genes expression in offspring rats
Xuan Zhang13, Ting‑yu Li13, Ke Chen2, Jie Chen3, You‑xue Liu3 and Ping Qu3
1Child Health Care, Children’s Hospital, Chongqing Medical University, Chongqing, P.R. China, 2Chengdu Maternal and Children Health Care Hospital, 32 Shiye Street, Chengdu, Sichuan Province, P.R. China, and 3Children’s Nutritional Research Center, Pediatric Research Institute, Chongqing Medical University, Chongqing, P.R. China
The present study was conducted to investigate the effects of marginal vitamin A deficiency (MVAD) beginning from pregnancy on hippocampus synaptic plasticity related gene expression in offspring rats. Sixteen female rats were randomly divided into control and MVAD groups. The dams and pups were fed with normal diet (VA 6500 IU/kg) and MVAD diet (VA 400 IU/kg) in control and MVAD group, respectively. Eight female pups were respectively killed at postnatal day 1 (P1d), P2w, P4w and P8w in two groups. Serum vitamin A (VA) concentration was monitored by high-performance liquid chromatography. The mRNA expressions of NMDA receptor (NR) subunits NR1, NR2A, NR2B, CAMK?α, Arc and CBP in hippocampus were detected by real time PCR. The serum VA concentration of MVAD group was significantly lower than that of control group at P8w (P<0.05). Real time-PCR results showed that mRNA levels of NR1 and NR2B in control group were significantly higher in most time points than those in MVAD group. The expression of Arc in control group was significantly higher than that in MVAD group at P4w and P8w (p<0.05), and CAMKIIα level in control group was higher than that in MVAD group at P2w and P4w (p<0.05). There was no difference of CBP level between the two groups. The data showed that MVAD beginning from pregnancy have influences on postnatal mRNA expressions of important genes related to synaptic plasticity pathway, such as NR1, NR2B, Arc and CAMKIIα.
Published online 1 August 2010, doi:10.1042/CBI034S026a
© The Author(s) Journal compilation © 2010 Portland Press Limited