|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2010) 34, S40 (Printed in Great Britain)
Mesenchymal stem cells enhances the viability of primary culture of rat hippocampal neurons under oxidative stress
Yang Liu12, Ting Ting Sun2, Xiao Hua Jiang1, Ting Yu Li2 and Hsiao Chang Chan1
1Epithelial Cell Biology Research Center, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, and 2Children’s Hospital, Chongqing Medical University, Chongqing, China
Mesenchymal stem cells (MSCs) are known to have pluripotency to differentiate into cells of various multi-lineages and their therapeutic potential in treatment of neuronal lesions and degenerative diseases has been proposed. Excessive oxidative stress is thought to be a key factor inducing brain damages and neuronal death, especially in the hippocampus. In this study, we co-cultured rat bone marrow MSCs with primary hippocampal neurons under H2O2-induced oxidative stress and the effect of MSCs on rescuing neuron death was evaluated. The primary hippocampal neurons of neonatal rat were cultured for seven days before H2O2 stress. Two concentrations of H2O2 were used (100μM and 250μM) and the treatment lasted for 2 hours. After oxidative stress the hippocampal neurons were co-cultured with MSCs (1:1) for 24 hours and the viable cells were then measured by MTS assay. 24 hours after 100μM and 250μM H2O2 treatment, the viable hippocampal neurons were significantly reduced to 48% and 34%, compare with the blank control group (p<0.05 and p<0.01, respectively). Co-culture with MSCs after the H2O2 treatments significantly increased the viable cells to 98% (p>0.05, vs co-culture control group) and 91% (p<0.05, vs co-culture control group). These data indicated that in either mild or severe oxidative stress conditions, MSCs could enhance the viability of hippocampal neurons in vitro. The underlying mechanisms, including the differentiation of MSCs into neurons and the release of anti-apoptotic factors from MSCs, are currently under investigation.
Published online 1 August 2010, doi:10.1042/CBI034S040a
© The Author(s) Journal compilation © 2010 Portland Press Limited