|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2010) 34, S43 (Printed in Great Britain)
Gender difference in FSHR positive cells derived from adult bone marrow stem cells induced by retinoic acid
Meiling Mi12, Bei Yang1, Mingdi Xiong1, Xiaying Lu1, Xiuli Ni1, Dalei Zhang1, Jiaxiang Chen1, Liusheng Li4, Xiaochun Yu3, Junhong Gao3 and Ting Zou1*
1Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, China, 4Library of Medical College, Medical College of Nanchang University, Nanchang, Jiangxi 330006, China, 2Genetic Laboratory of Cangzhou Maternal and Child Care Hospital, Cangzhou, Hebei 061000, China, and 3Institute of Acupuncture & Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China
The aim is to investigate whether adult male and female bone marrow stem cells (BMSCs) are able to differentiate into follicle-stimulating hormone receptor (FSHR) positive Sertoli cells (SCs)-like and granulosa cells (GCs)-like cells respectively in vitro induced by all-trans retinoic acid (RA) and to compare the sex difference. Male and female BMSCs from adult male or female SD rat bone marrow were cultured respectively in DMEM supplemented with 10% fetal bovine serum. The BMSCs were cultured above medium with 10-6 M RA or without RA (control group) for 7 days. Two days after induction culture, some of the male BMSCs began to expresse FSHR, which is a SCs specific marker in the testicular cells, by immunocytochemistry staining and RT-PCR. The cells were middle in size and had elongated spindle appearance. Meanwhile some of the female BMSCs were also FSHR positive, which is a GCs specific marker in the ovary cells. The cells were small or medium sized, most of them presented spindle and a few cells were round in shape. Most of these FSHR positive cells derived from male and female BMSCs were found in small clusters. Following longer in culture (from day 5 to day 7), a good few male FSH positive cells gradually changed their shape from spindle to polygonal or triangular shape, the most of female FSH positive cells gradually became round and were larger. In control group, the BMSCs still kept undifferentiated relatively elongated or spindle-shaped cells that they were FSHR negative. These results suggest that RA could induce Male and female BMSCs to differentiate into FSHR positive SC-like and GC-like cells respectively in vitro. It is dependent on the BMSCs sex on our experimental condition. This research was supported by National Natural Science Foundation of China Grant 30960409.
Published online 1 August 2010, doi:10.1042/CBI034S043a
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