|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2010) 34, S46 (Printed in Great Britain)
Suppression of RBP-WH inhibits cell cycle progression and induces apoptosis in glioma cells
Wei Han, Zhongshuai Xin, Bin Yin, Ping Li, Boqin Qiang, Jiangang Yuan and Xiaozhong Peng
The National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China
Human RNA binding proteins (RBPs) have been reported to be involved in human malignancies and several RBPs have been found as promising biomarkers of lung, head and neck, colon, breast, and pancreatic cancers. The present study investigated the biological significance of RBP-WH in human gliomas. Human RBP-WH was significantly up-regulated in glioma tissues and cell lines compared to normal brain tissues as determined by Real-time PCR and Western Blot. Transient transfection siRNA of RBP-WH in human glioma cell lines resulted in inhibition of cell cycle progression and induction of apoptosis of cells. Compared to negative controls, cells with knockdown of RBP-WH expression showed a G1/S arrest by flow cytometry and an increase in the number of apoptotic cells by TUNEL in situ cell death detection kit-POD assay. Furthermore, employing the RIP-Chip assay we consistently identified a group of 35 mRNAs in three independent analyses of RBP-WH-RNP complexes biotin-purified from a human glioma cell line (T98G). In our RIP-Chip analysis we found that 25% of the RBP-WH associated mRNAs (9 genes) were involved in regulation of cell cycle and apoptosis. These results suggest that RBP-WH affects a network of genes and could function as a master regulator during glioma development and formation.
Published online 1 August 2010, doi:10.1042/CBI034S046c
© The Author(s) Journal compilation © 2010 Portland Press Limited