|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2010) 34, S49 (Printed in Great Britain)
Human cervical cancer CaSki cells adapted to stimuli by regulating the expression of genes
Feng‑Jie Guo, Ya‑Lin Li, Jia‑Jia Wang, Yue‑Hui Li, Yan‑Hong Liu, Jie Song, Feng Li and Guan‑Cheng Li*
Laboratory of Tumor Immunobiology, Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha, Hunan 410078, PR China
Until now, cancer is still difficult to treat, while the difficulty of curing tumor is associated with the response of tumor cell to stimulus. To understand the molecular mechanism of tumor cell responding to stimulus, human cervical carcinoma Caski cells were flown on “Shen Zhou IV”space shuttle mission. The electron microscope was used to observe the morphological character in ground and space cervical cancer CaSki cells. Reverse Northern blot and RT-PCR were applied to exam differentially expressed genes in cervical on ground and in space cancer CaSki cells using the previous constructed cDNA library. RT-PCR was used to check the expression of the genes while the CaSki cells were treated with drugs, heat, and rays. We found that the ultrastructure of space cervical cancer CaSki cells changed compared with ground group, including large mitochondrion, high density of cytoplasm, enhanced ribosome, and wide microtubule. We obtained 35 differentially expressed genes which were associated with cytoskeleton, apoptosis, cell cycle, transcription, and immune. The genes accordingly altered when CaSki cells were treated with drugs, heat, and rays. In conclusion, the survival CaSki cells adapted to the change of environments by regulating the expression of genes and then changing cell morphous, when the outside conditions changed. Thus, these genes formed a protective network to react quickly in the face of external stimuli. This study was support by National Natural Science Foundation of China, No. 30672352 and Innovation Fund of Central South University (2340-74334000006).
Published online 1 August 2010, doi:10.1042/CBI034S049b
© The Author(s) Journal compilation © 2010 Portland Press Limited