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Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
Cell Biology International (2010) 34, S64 (Printed in Great Britain)
Meeting Abstract
Selection of human stem cell factor mimetic peptides from phage-displayed random peptide library
Lin SU1Δ, Yan KONG2Δ, Chang‑zheng LIU1, Pei‑chen JIA1, Ke‑gong YANG1 and Song‑sen CHEN1*
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China, and 2Cancer and Melanoma, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing 100036, China

The hematopoietic growth factors (HGFs) control the proliferation and differentiation of blood stem cells and progenitor cells and play a role in modulating the immune system to some degree. Mimetic peptides of human EPO and human TPO had been generated respectively in 1990s. However, mimetic peptides of human stem factor cell factor (hSCF) have not been reported. Here, we described that screening phage clones with high hSCF receptor (rc-kit/Ig1-3) binding activity from phage-display random hepta/dodeca peptide library by using phage ELISA assay, abstracting and sequencing phage single DNA was carried out. 11 ph.D-C7C clones and 8 ph.D-12 phage clones with high binding activity with hSCF receptor were selected. Sequence analysis showed that there were no consensus sequence between hSCF and these screened mimetic peptides, except one consensus sequence DPSPHTH found in heptapeptid library. Four kinds of peptides (CE3, CE16, LE4 and LE20) with higher c-kit/Ig1-3 binding activity were chemically synthesized and characterized by using cell proliferation assay with MTT in UT-7 cells. These four kinds of synthesized peptides could stimulate UT-7 cell proliferation shown by MTT assay, especially CE16 and LE20. These results illustrated that four kinds of hSCF mimetic peptides were successfully isolated from phage-displayed random peptide library, laying a foundation for probing the function of hSCF mimetic peptides and clinical application.

ΔThese authors contributed equally to this work

*correspondence author

Published online 1 August 2010, doi:10.1042/CBI034S064d

© The Author(s) Journal compilation © 2010 Portland Press Limited

ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)