Download to Citation Manager

Cell Biology International (2010) 34, S75 (Printed in Great Britain)

PDF

Add a comment

Citing articles

Meeting Abstract

Preliminary Research of the aldosterone synthesized by Bone Morrow Stem Cells of adult male rats in vitro

Wei‑ying Zou, Bei Yang, Xiuli Ni, Da‑lei Zhang, Lei Wu, Jing‑lei Wang and Ting Zou*

Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, P.R. China

Aldosterone plays an important role in salt and water homeostasis and blood pressure control. Recently systemic or local aldosterone has emerged as a multifunctional hormone exhibiting profibrotic and proinflammatory actions. The renin-angiotensin-aldosterone system (RAAS) is central to the pathogenesis of hypertension, cardiovascular disease, and kidney disease. The variability in effects of RAAS on cardiovascular progenitor cells might reflect differences between the various models with respect to circulating and local tissue RAAS activation. The aim was to investigate whethere the bone marrow stem cells (BMSCs) are involved in the local tissue RAAS activation. Methods: The BMSCs were isolated from male rat bone mallow and cultured in the DMEM containing 10% fetal bovine serum (FBS). The primary BMSCs from the adherent cell fraction were cultured for 21 days. The whole medium was replaced 2 times every week and was then collected. The levels of aldosterone in the media were measured by radioimmunoassay (RIA). Results: The concentration of aldosterone in the culture medium on day 0 was low (M±SD, 16.472±11.594 pg/ml). The aldosterone content in the culture medium on day 7 was 52.718±37.162 pg/ml (P>0.05). The higher levels of aldosterone in the culture media were detected by RIA on days 11 (125.46 ± 17.439 pg/ml, P<0.01); 14 (115.012 ± 24.200 pg/ml, P<0.01); 18 (133.028 ± 11.141 pg/ml, P<0.01) and 21 (99.982 ± 34.423 pg/ml, P<0.01). In conclusion, the BMSCs of adult male rats can synthesize aldosterone in vitro. The results suggest that the BMSCs might be involved in the local RAAS and its role need to be explored in a further step of the research work. The research was supported by National Natural Science Foundation of China Grant 30960409.

^*correspondence author

Published online 1 August 2010, doi:10.1042/CBI034S075d

© The Author(s) Journal compilation © 2010 Portland Press Limited