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Figure 7 Immunocytochemical analysis of GDNF/LIF-treated tsAM5NE cells grown at the permissive and non-permissive temperatures
tsAM5NE cells were treated with a combination of GDNF (50 ng/ml) and LIF (25 ng/ml) in serum-containing medium for 3 days at either 33 (A, C and E) or 39°C (B, D and F) and then processed for immunocytochemical analysis. (A and B) The results of double-immunofluorescence staining for tsSV40T (red) and TH (green). The cells were double-stained with rabbit polyclonal TH and mouse monoclonal SV40T antibodies followed by Alexa488-conjugated donkey anti-rabbit IgG and Alexa568-conjugated goat anti-mouse IgG antibodies and inspected by confocal fluorescence microscopy. (C and D) Cells stained immunofluorescently for MAP-2. The cells were stained with rabbit polyclonal MAP-2 antibody followed by Alexa488-conjugated donkey anti-rabbit IgG antibody and inspected by confocal fluorescence microscopy. (E and F) Immunofluorescence staining results for NF160K. The cells were stained with mouse monoclonal NF160K antibody followed by Alexa488-conjugated donkey anti-mouse IgG antibody and observed by confocal fluorescence microscopy.