Standard resolution | High resolution

Figure 4 Cytoskeleton compounds detected by fluorescence in murine peritoneal macrophages exposed to 50 μg/ml KA for 1 h

(a–f) Fluorescence labelling of actin filaments with phalloidin and DAPI in untreated cells (a–c), KA-treated macrophages (d–f) with enhanced filopodium establishment (small arrows); (g–l) fluorescence labelling of microtubules with polyclonal anti-tubulin antibody and DAPI in untreated cells (g–i), KA-treated macrophages (j–l) with microtubule polymerization extending from the nucleus membrane to the cell membrane (arrows); (m–r) immunofluorescence labelling of intermediate filaments (vimentin) with polyclonal anti-vimentin antibody and DAPI in untreated cells (m–o), KA-treated macrophages (p–r) with greater distribution of vimentin on the macrophage surface (thin arrows); Insets: negative control of Alexa594-labelled goat anti-rabbit IgG; Bars: 10 μm.