Standard resolution | High resolution



Figure 4 RT–PCR analysis of SPC transcripts

(A) Samples from left to right are: BMSCs in growth medium, MRC-5 in growth medium, BMSCs in SAGM, MRC-5 in SAGM, BMSCs co-cultured with MRC-5 in growth medium and lung tissue. (B) SPC gene expression in BMSCs co-cultured with MRC-5 at day 5, 10, 15, 20 and 25 respectively. (C) Relative amounts of SPC transcripts from (B), which was calculated as the ratio of SPC mRNA to β-actin mRNA. **P<0.01, ***P>0.05. (D) Upper panel, SPC gene expression in BMSCs co-cultured with MRC-5 on day 15 at different ratio. Lower panel, relative amounts of SPC transcripts, which was calculated as the ratio of SPC mRNA to β-actin mRNA. #P<0.05, *P>0.05. (F) Upper panel, SPC gene expression in BMSCs co-cultured with MRC-5 on day 15 in SAGM and modified SAGM. Lower panel, relative amounts of SPC transcripts, which was calculated as the ratio of SPC mRNA to β-actin mRNA. P<0.05.


Cell Biology International (2011) Volume 35, 1261-1266