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Cytoplasmic and nuclear localization of cadherin in honey bee (Apis mellifera L.) gonads
Mônica M Florecki1 and Klaus Hartfelder
Departamento de Biologia Celular e Molecular e Bioagentes Patognicos, Faculdade de Medicina de Ribeiro Preto, Universidade de So Paulo, Ribeiro Preto, SP, Brazil
1To whom correspondence should be addressed (email firstname.lastname@example.org).
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SUPPLEMENTARY ONLINE DATA
Figure S1 Cadherin detected by Western blot analysis in ovaries of adult honeybee queens and in testes of drone larvae
A cadherin-immunoreactive protein of slightly over 300 kDa was detected in both ovary and testis homogenates. In ovary samples, the antibody also reacted with a protein of approximately 180 kDa (markers: 170 and 220 kDa) which, according to molecular mass, could be vitellin, thus representing unspecific binding to a prominent ovarian protein.
Table S1 Gene-specific primers for the amplification of five A. mellifera cadherins
Primer design was based upon the genomic predictions of cadherin gene family members.
aProduct size in base pairs. bMelting temperature.
aProduct size in base pairs.
Received 18 May 2010/29 June 2010; accepted 24 August 2010
Published as Cell Biology International Immediate Publication 24 August 2010, doi:10.1042/CBI20100333
© The Author(s) Journal compilation © Portland Press Limited