|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Stem cell-conditioned medium does not protect against kidney failure
Yousof Gheisari*1, Naser Ahmadbeigi*†1, Mahmood Naderi*, Seyed Mahdi Nassiri‡, Samad Nadri* and Masoud Soleimani§2
*Department of Stem Cell Biology and Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran, †SABZ Biomedicals, Tehran, Iran, ‡Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, and §Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
1Yousof Gheisari and Naser Ahmadbeigi contributed equally to this work.
2To whom correspondence should be addressed (email firstname.lastname@example.org).
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Figure S1 Characterization of mouse bone marrow mesenchymal stem cells
(A) Oil Red O staining determined differentiation to adipocytes. (B) Alcian Blue indicated differentiation to chondrocytes. (C) Alizarin Red S was used to show differentiation to osteocytes. (D) Alkaline phosphatase staining also confirmed differentiation to osteocytes. (E) Representative flow cytometry graphs of surface markers. The cells were positive for CD29, CD44, CD73, CD90 and CD105 and negative for CD45, CD11b, VEGFR2, CD31 and CD34. Black areas, tested antibodies; grey areas, isotype controls; scale bars, 200 μm.
Published as Cell Biology International Immediate Publication 18 October 2010, doi:10.1042/CBI20100183
© The Author(s) Journal compilation © 2011 Portland Press Limited