|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Effect of carbon nanotube coating of aligned nanofibrous polymer scaffolds on the neurite outgrowth of PC-12 cells
Guang‑Zhen Jin*, Meeju Kim*†, Ueon Sang Shin* and Hae‑Won Kim*†‡1
*Institute of Tissue Regeneration Engineering ITREN, Dankook University, Cheonan 330714, South Korea, †Department of Nanobiomedical Science and WCU Research Center, Dankook University Graduate School, Cheonan 330714, South Korea, and ‡Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330714, South Korea
Neurite outgrowth from endogenous or transplanted cells is important for neural regeneration following nerve tissue injury. Modified substrates often provide better environments for cell adhesion and neurite outgrowth. This study was conducted to determine if MWCNT (multiwalled carbon nanotube)-coated electrospun PLCL [poly (l-lactic acid-co-3-caprolactone)] nanofibres improved the neurite outgrowth of PC-12 cells. To accomplish this, two groups, PC-12 cells in either uncoated PLCL scaffolds or MWCNT-coated PLCL scaffolds were cultured for 9 days. MWCNT-coated PLCL scaffolds showed improved adhesion, proliferation and neurite outgrowth of PC-12 cells. These findings suggest that MWCNT-coated nanofibrous scaffolds may be an attractive platform for cell transplantation application in neural tissue engineering.
Key words: aligned nanofibre, carbon nanotube, neurite outgrowth, PC-12 cell, PLCL
Abbreviations: CCK-8, cell counting kit-8, CNTs, carbon nanotubes, DIV, days in vitro, DMEM, Dulbecco's modified Eagle's medium, FBS, fetal bovine serum, MWCNTs, multiwalled CNTs, NGF, nerve growth factor, PLCL, poly (l-lactic acid-co-3-caprolactone), SEM, scanning electron microscopy, TGA, thermogravimetric analysis
1To whom correspondence should be addressed (email firstname.lastname@example.org).
Received 27 September 2010/20 January 2011; accepted 21 February 2011
Published as Cell Biology International Immediate Publication 21 February 2011, doi:10.1042/CBI20100705
© The Author(s) Journal compilation © 2011 Portland Press Limited