|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Adipogenic differentiation and EGFP gene transfection of amniotic fluid-derived stem cells from goat fetus at terminal gestational age
Xiao‑Ying He1, Yue‑Mao Zheng12, Shuang Qiu, Ying‑Pei Qi and Yong Zhang
College of Veterinary Medicine, Northwest AF University, Yangling, Shaanxi 712100, Peoples Republic of China
The aims of this study were to determine whether stem cells could be isolated from amniotic fluid of goat fetus at terminal gestational age and to determine if these stem cells could differentiate into adipogenic cells and be transfected with a reporter gene, EGFP (enhanced green fluorescent protein). The stem cells were isolated from amniotic fluid of goat fetus at terminal gestational age, induced to differentiate into adipogenic cells in vitro and transfected with the EGFP gene using lipofection. Markers associated with undifferentiated AFS (amniotic fluid-derived stem) cells were tested by RT (reverse transcription)-PCR. The results demonstrated that AFS cells could be isolated from amniotic fluid of goat fetus at terminal gestational age and could differentiate into adipogenic cells. The EGFP gene was transfected into AFS cells successfully. EGFP gene transfection efficiency of the three groups of transgenic AFS cells were 26.0, 29.9 and 30.5%, respectively. Both transgenic and wild-type AFS cells could express Hes1 (hairy and enhancer of split 1), Oct4 (octamer-binding protein 4) and Nanog.
Key words: adipogenic differentiation, amniotic fluid-derived stem cell, EGFP gene transfection, goat
Abbreviations: AFS, amniotic fluid-derived stem, DMEM, Dulbecco's modified Eagle's medium, EGFP, enhanced green fluorescent protein, ES, embryonic stem, Hes1, hairy and enhancer of split 1, Oct4, octamer-binding protein 4, RT, reverse transcription
1Xiao-Ying He and Yue-Mao Zheng contributed equally to this work.
2To whom correspondence should be addressed (email firstname.lastname@example.org).
Received 13 November 2010/11 January 2011; accepted 19 January 2011
Published as Cell Biology International Immediate Publication 19 January 2011, doi:10.1042/CBI20100811
© The Author(s) Journal compilation © 2011 Portland Press Limited