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Retention and transmission of active transcription memory from progenitor to progeny cells via ligand-modulated transcription factors: elucidation of a concept by BIOPIT model
Sanjay Kumar, Mallampati Saradhi, Nagendra K. Chaturvedi and Rakesh K. Tyagi1
Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110067, India
1To whom correspondence should be addressed (email email@example.com or firstname.lastname@example.org).
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Figure S1 Retention and transmission of active transcription memory from progenitor to progeny cells via ligand-modulated transcription factors
Images demonstrate agonist-mediated generation of nuclear foci and their migration with the condensed chromosomes during all the stages of mitosis in a human cell line HEK-293T. HEK-293T cells were transiently transfected with GFP–AR and incubated in steroid-free medium for 24 h. Following receptor expression the cells were treated with either DMSO/ethanol (vehicle) for control cells or with DHT. After incubation period, live cells expressing GFP–AR were recorded by fluorescence microscopy. Left panels (−DHT) shows unliganded AR that does not associate with the condensed chromosomes during mitosis. Right panels (+DHT) shows DHT-activated AR that binds to the condensed chromosomes during all the stages of mitosis. The DNA stain Hoechst (blue) was used to visualize the corresponding nuclei/condensed chromosomes.
Received 23 October 2009/10 June 2011; accepted 18 October 2011
Published as Cell Biology International Immediate Publication 18 October 2011, doi:10.1042/CBI20090329
© The Author(s) Journal compilation © 2012 Portland Press Limited