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Cell Biology International (2012) 36, 177–182 (Printed in Great Britain)

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Retention and transmission of active transcription memory from progenitor to progeny cells via ligand-modulated transcription factors: elucidation of a concept by BIOPIT model

Sanjay Kumar, Mallampati Saradhi, Nagendra K. Chaturvedi and Rakesh K. Tyagi¹

Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110067, India

¹To whom correspondence should be addressed (email rktyagi@yahoo.com or rkt2300@mail.jnu.ac.in).

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Figure S1 Retention and transmission of active transcription memory from progenitor to progeny cells via ligand-modulated transcription factors

Images demonstrate agonist-mediated generation of nuclear foci and their migration with the condensed chromosomes during all the stages of mitosis in a human cell line HEK-293T. HEK-293T cells were transiently transfected with GFP–AR and incubated in steroid-free medium for 24 h. Following receptor expression the cells were treated with either DMSO/ethanol (vehicle) for control cells or with DHT. After incubation period, live cells expressing GFP–AR were recorded by fluorescence microscopy. Left panels (−DHT) shows unliganded AR that does not associate with the condensed chromosomes during mitosis. Right panels (+DHT) shows DHT-activated AR that binds to the condensed chromosomes during all the stages of mitosis. The DNA stain Hoechst (blue) was used to visualize the corresponding nuclei/condensed chromosomes.

Received 23 October 2009/10 June 2011; accepted 18 October 2011

Published as Cell Biology International Immediate Publication 18 October 2011, doi:10.1042/CBI20090329

© The Author(s) Journal compilation © 2012 Portland Press Limited