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Cell Biology International (2012) 36, 183–188 (Printed in Great Britain)

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Cytoprotective effect of trolox against oxidative damage and apoptosis in the NRK-52e cells induced by melamine

Chengzhi Guo*, Zuping He†‡¹, Lixin Wen*, Li Zhu*, Yin Lu*, Sijun Deng*, Yang Yang*, Qiang Wei* and Hui Yuan*¹

*College of Veterinary Medicine, Hunan Agricultural University, 19 East Lake Road, Changsha, Hunan 410128, Peoples Republic of China, †Clinical Stem Cell Research Center, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 1630 Dongfang Road, Shanghai 200127, Peoples Republic of China, and ‡Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, 3900 Reservoir Road NW, Washington, DC 20057, U.S.A.

¹Correspondence may be addressed to either of these authors (email zupinghe@gmail.com or yuanhui7269@yahoo.com.cn).

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Figure S1 Proliferation assays of the NRK-52e cells with or without various concentrations of melamine and trolox treatment

The NRK-52e cells were seeded at a density of 5×10⁴ cells/ml on 96-well plates. The cells were pre-treated with 0, 0.5, 1, 5 or 10 mM trolox for 30 min and then treated with 0, 8, 16 or 24 mM melamine. Proliferation assays of NRK-52e cells were performed by measuring absorbance at wavelength of 570 nm using a microplate reader according to the procedure as described previously (Mosmann, 1983; Zhang et al., 2005; Zhang et al., 2010).

REFERENCES

Mosmann, T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65, 55-63
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Zhang, H, Miao, Z, He, Z, Yang, Y, Wang, Y and Feng, M (2005) The existence of epithelial-to-mesenchymal cells with the ability to support hematopoiesis in human fetal liver. Cell Biol Int 29, 213-9
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Zhang, M, He, Z, Wen, L, Wu, J, Yuan, L and Lu, Y (2010) Cadmium suppresses the proliferation of piglet Sertoli cells and causes their DNA damage, cell apoptosis and aberrant ultrastructure. Reprod Biol Endocrinol 8, 97
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Received 16 January 2011/23 May 2011; accepted 22 September 2011

Published as Cell Biology International Immediate Publication 22 September 2011, doi:10.1042/CBI20110036

© The Author(s) Journal compilation © 2012 Portland Press Limited