|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Wnt3a is involved in the early stage of miPSC and mESC haemopoietic differentiation
Wencheng Zhang, Hailei Yao, Sihan Wang, Shuangshuang Shi, Yang Lv, Lijuan He, Xue Nan, Wen Yue, Yanhua Li1 and Xuetao Pei1
Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, Peoples Republic of China
1Correspondence may be addressed to either of these authors (email firstname.lastname@example.org or email@example.com).
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SUPPLEMENTARY ONLINE DATA
Figure S1 Constructing and packaging of pBPLV-Wnt3a plasmid
Top panels, generation of the lentiviral plasmid with Wnt3a. Wnt3a gene sequence was inserted into GFP-labelled pLentiviral vector in the Xba-1 enzyme site. Bottom panels, virus packaging of pBPLV-Wnt3a pLentiviral vector in 293FT cells. GFP expression reflected virus production in the packaging cells. Scale bar = 150 μm.
Figure S2 Generating Wnt3a-OP9 cell line through LV-Wnt3a infection
Left-hand panels, Wnt3a-OP9 cell line was generated using LV-Wnt3a infection. The cells were then sorted according to the expression of GFP. Scale bar = 100 μm. Right-hand panel, RT–PCR results for the expression of Wnt3a gene in Wnt3a-OP9 cells in contrast with control OP9 cells.
Table S1 Description of primers used for RT–PCR analysis
Received 30 October 2010/22 August 2011; accepted 17 October 2011
Published as Cell Biology International Immediate Publication 17 October 2011, doi:10.1042/CBI20100766
© The Author(s) Journal compilation © 2012 Portland Press Limited