|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Improvement of the nuclear transfer efficiency by using the same genetic background of recipient oocytes as the somatic donor cells in goats
Hai‑jun Liu*1, Jun Xue†, Kui Li‡, Zheng‑Zhou Ying‡, Zi Zheng* and Ru Wang*
*Tianjin Institute of Animal Science and Veterinary Medicine, Tianjin 300112, Peoples Republic of China, †Tianjin Research Center of Agricultural Biotechnology, Tianjin 300192, Peoples Republic of China, and ‡Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100094, Peoples Republic of China
We have compared the effect of the genetic background of recipient oocytes on the in vitro and in vivo development of nuclear transfer reconstructed embryos in goats. Adult fibroblast cells from Boer goats were used as donor cells, and recipient oocytes were obtained from Boer goats and Boer cross-breeds (Boer♂×Huanghuai♀). Nuclear transfer reconstructed embryos were cultured in vitro, or transferred into recipient goats. The mitochondrial origin of 2 cloned Boer goats was investigated by analysing the D-loop region based on polymorphisms via DNA sequencing. There was no significant difference in the fusion rate and cleavage rate of reconstructed embryos (P>0.05), when using Boer and cross-breeding goat oocytes as recipient cytoplast respectively. However, in vitro morula development of reconstructed embryos from Boer oocytes was significantly higher than that of cross-breeding embryos (34.1% versus 19.1%, P<0.05). There was no significant difference in the rate of pregnancy and foetus loss between the 2 breeds. However, the live-birth rate was significantly higher with Boer goat oocyte recipients than the cross-breeds (3.1% versus 0.8%, P<0.05). Mitochondrial analysis showed that the 2 cloned goats were similar to their respective oocyte donor goats, and significantly different from the nucleus donor. In conclusion, genetic background of recipient oocytes affected in vitro and in vivo development of reconstructed embryos, with the homologous background of cytoplast and nuclear donor benefiting development of reconstructed embryos. The mitochondrial origin of the 2 cloned Boer goats came from recipient oocytes, not donors.
Key words: genetic background, goat; mitochondria origin, nuclear–cytoplasmic interactions, nuclear transfer, oocyte donor
Abbreviations: FCS, foetal calf serum, FSH, follicle-stimulating hormone, I.U., international units, mtDNA, mitochondrial DNA
1To whom correspondence should be addressed (email firstname.lastname@example.org).
Received 25 May 2011/18 January 2012; accepted 23 February 2012
Published as Cell Biology International Immediate Publication 23 February 2012, doi:10.1042/CBI20110287
© The Author(s) Journal compilation © 2012 International Federation for Cell Biology