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Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
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Cell Biology International (2012) 36, 589–594 (Printed in Great Britain)
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Role of bone marrow-derived mesenchymal stem cells in the prevention of hyperoxia-induced lung injury in newborn mice
Xiaoying Zhang*12, Hui Wang†1, Yun Shi*, Wei Peng*, Sheng Zhang*, Wanqiao Zhang*, Jing Xu*, Yabo Mei* and Zhichun Feng*2
*Department of Pediatrics, BaYi Childrens Hospital of The General Military Hospital of Beijing PLA, Beijing 100700, Peoples Republic of China, and †Department of Science Division, The General Military Hospital of Beijing PLA, Beijing 100700, Peoples Republic of China

1These authors have contributed equally to this work.

2Correspondence may be addressed to either of the authors (email fengzhichun81@sina.com or xiaoyingzhang79@163.com).

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Figure S1 Morphology, immunophenotype and differentiation analysis of BMSC

(A) Morphology of BMSC at passages 1 and 2. At passage 1, most cells were elongated with a typical spindle, while some cells had a polygonal morphology. At passage 2, the cells displayed the typical homogeneous morphology of BMSC (scale bar = 100 μm). (B) Immunophenotype of BMSC at passage 2. Shown were the fluorescence intensity histograms for antibodies specific to membrane antigens (black line) and irrelevant isotypic-matched antibody used as a negative control (red line). (C) Differentiation of BMSC at passage 2 after culturing in differentiation induction medium (scale bar = 50 μm).

Table S1 Primers for SP-C, TGFβ1, collagen 1α, TIMP-1 and GAPDH

Name Sequence
SP-C F: 5′-GCCTTCTCATCGTGGTTGT -3′
R: 5′-CCAGTATCATGCCCTTCCT-3′
TGFβ1 F: 5′-GGCGGTGCTCGCTTTGTAC-3′
R: 5′-TCCCGAATGTCTGACGTATTGA-3′
Collagen 1α F: 5′-TTGACCCTAACCAAGGATGC-3′
R: 5′-CACCCCTTCTGCGTTGTATT-3′
TIMP-1 F: 5′-TCCCCAGAAATCAACGAGACCACCT-3′
R: 5′-AGAGTACGCCAGGGAACCAAGAAGC-3′
GAPDH F: 5′-TTCACCACCATGGAGAAGGC-3′
R: 5′-GGCATGGACTGTGGTCATGA-3′


Received 11 September 2011/18 January 2012; accepted 20 February 2012

Published as Cell Biology International Immediate Publication 20 February 2012, doi:10.1042/CBI20110447

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ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
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