|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Combination effect of PectaSol and Doxorubicin on viability, cell cycle arrest and apoptosis in DU-145 and LNCaP prostate cancer cell lines
Najmeh Tehranian*, Houri Sepehri*1, Parvin Mehdipour‡, Firouzeh Biramijamal§, Arash Hossein‑Nezhad¶‖, Abdolfattah Sarrafnejad‖ and Ebrahim Hajizadeh**
*Animal Biology Department, School of Biology, University College of Sciences, University of Tehran, PO Box 1415, Tehran, Islamic Republic of Iran, ‡Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, PO Box 1417613151, Tehran, Islamic Republic of Iran, §National Institute of Genetic Engineering and Biotechnology, PO Box 14965161, Tehran, Islamic Republic of Iran, ¶Bio and Nanotechnology group, Endocrinology and Metabolism Research Center, Shariati Hospital, Tehran University of Medical Sciences, PO Box 14965161, Tehran, Islamic Republic of Iran, ‖Department of Pathology, School of Public Health, Tehran University of Medical Sciences, PO Box 1417613151, Tehran, Islamic Republic of Iran, and **Department of Biostatistics, Faculty of Medical Sciences, Tarbiat Modares University, PO Box 14115111, Tehran, Islamic Republic of Iran
The effect of PectaSol on Dox (Doxorubicin) cytotoxicity in terms of apoptosis and cell cycle changes in PCa (prostate cancer) cell lines (DU-145 and LNCaP) has been investigated. Combination of PectaSol and Dox resulted in a viability of 29.4 and 32.6% (P<0.001) in DU-145 and LNCaP cells. The IC50 values decreased 1.5-fold and 1.3-fold in the DU-145 and LNCaP cells respectively. In the DU-145 cells, combination of PectaSol and Dox resulted in a reduction in p27 gene and protein expression (P<0.001). In LNCaP cells, this combination increased p53, p27 and Bcl-2 expression. Treatment with both drugs in DU-145 cells led to an increase in sub-G1 arrest (54.6% compared with 12.2% in Dox). In LNCaP cells, combination of the drugs led to an increased in G2/M arrest (61.7% compared with 53.6% in Dox). Based on these findings, progressive cytotoxicity effect of Dox and PectaSol together rapidly induce cell death in DU-145 through apoptosis and in LNCaP cells through cell cycle arrest (G2/M arrest).
Key words: Doxorubicin (Dox), gene expression, PectaSol, prostate cancer (PCa) cell lines (DU-145, LNCaP), protein expression, viability
Abbreviations: Dox, Doxorubicin, MCP, modified citrus pectin, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide, PTEN, phosphatase and tensin homologue deleted on chromosome 10, RT-PCR, real-time PCR
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Received 25 May 2011/19 September 2011; accepted 4 January 2012
Published as Cell Biology International Immediate Publication 4 January 2012, doi:10.1042/CBI20110309
© The Author(s) Journal compilation © 2012 International Federation for Cell Biology