|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Genes expression profiling of peripheral blood cells of patients with hepatocellular carcinoma
Peng‑jun Zhang*†¶1, Run Wei*¶1, Xin‑yu Wen*¶1, Liang Ping§, Cheng‑bin Wang‡, Zhen‑nan Dong*¶, Xin‑xin Deng*¶, Wang Bo*†¶, Chen Bin*†¶ and Ya‑ping Tian*†¶2
*Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, People's Republic of China, †Medical College, Nankai University, Tianjin, People's Republic of China, ‡Department of Clinical Laboratory, Chinese PLA General Hospital, Beijing, People's Republic of China, §Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing, People's Republic of China, and ¶State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, Beijing, People's Republic of China
HCC (hepatocellular carcinoma) is often diagnosed at an advanced stage with poor prognosis. Peripheral blood may be useful in cancer classification, and therefore we investigated the gene expression found by Affymetrix HG-U133 Plus2.0 microarray, with samples from nine HCC patients and five healthy NC (normal controls). A total of 726 probe sets showed significant differences based on the criteria of P<0.05 and absolute fold change >2. The genes were related to many biological functions, including immune response, transcription regulation and metabolism processes. Ten genes [IL-8 (interleukin 8), GOS2 (G0/G1 switch gene 2), CXCR4 (CXC chemokine receptor 4), FOS, RPS24 (40S ribosomal protein S24), HAP90AA1, PFDN5, RPL27, GZMA and PFN1] showing significant differences were confirmed by real-time PCR in 54 HCC patients and 56 healthy NC. Seven genes [IL-8, GOS2, CXCR4, FOS, RPS24, HSP90AA1 (heat shock protein 90AA1) and PFN1] showed significant difference both in RT–PCR (reverse transcription–PCR) and microarray. Expression of IL-8 and FOS proteins was up-regulated in HCC compared with healthy controls. A gene signature in peripheral blood which can distinguish HCC patients and healthy controls may have been identified.
Key words: gene, hepatocellular carcinoma (HCC), microarray, peripheral blood
Abbreviations: CXCR4, CXC chemokine receptor 4, GAPDH, glyceraldehyde-3-phosphate dehydrogenase, GOS2, G0/G1 switch gene 2, HBV, hepatitis B virus, HCC, hepatocellular carcinoma, HSP, heat shock protein, IL-8, interleukin 8, NC, normal controls, RT–PCR, reverse transcription–PCR, RPS24, 40S ribosomal protein S24
1These authors have contributed equally to this work.
2To whom correspondence should be addressed (email firstname.lastname@example.org).
Received 29 December 2010/16 February 2012; accepted 14 May 2012
Published as Cell Biology International Immediate Publication 14 May 2012, doi:10.1042/CBI20100920
© The Author(s) Journal compilation © 2012 International Federation for Cell Biology